Which of the following is a method used to insert or transform cells with a plasmid?
A)add the DNA to bacterial cells that have been lightly treated with lysozyme to produce "holes" in the cell wall
B)add the DNA to a heated suspension of cells at 42°C
C)treat the bacteria with Ca²⁺,add the DNA,and briefly heat to 42°C
D)incubate the DNA with the cells overnight at 4°C

Question

Quizplus · Accepted Answer

This method is called the calcium chloride method, and it involves treating the bacteria with calcium ions to make them more permeable to DNA, then adding the DNA and briefly heating the cells to 42°C to encourage uptake of the plasmid. This method is commonly used in molecular biology for genetic manipulation of bacteria. Option A is known as electroporation, which involves using electric pulses to create temporary pores in the cell membrane. Option B is incorrect as 42°C is not the optimal temperature for transformation, and heating up the cells can damage them. Option D is also incorrect as incubating at 4°C is too cold to facilitate transformation.

Which of the Following Is a Method Used to Insert