Which of the following steps is NOT involved with each cycle of amplification in PCR?
A)the addition of fresh dNTPs to the reaction mixture
B)annealing of oligodeoxyribonucleotide primers to DNA
C)thermal denaturation of the target duplex DNA
D)reaction with DNA polymerase at approximately 70°C

Question

Quizplus · Accepted Answer

The addition of fresh dNTPs to the reaction mixture is not required with each cycle of PCR. dNTPs are added in excess at the beginning of the PCR process and are not replenished in each cycle.

Which of the Following Steps Is NOT Involved with Each