What would be the 9-residue primer used to amplify this sequence?
5'-ATCGACGTTACGCTACATAGCATAAGGCTT-3'
A)5'-TAGCTGCAA-3'
B)5'-AAGCCTTAT-3'
C)5'-UAGCUGCAA-3'
D)5'-AAGCCUUAU-3'

Question

Quizplus · Accepted Answer

A 9-residue primer would be 5'-AAGCCTTAT-3'. This primer would match the sequence at the 11th through 19th positions, which is the correct length for a 9-residue primer. Choice A is incorrect because it only includes 8 nucleotides. Choice C is incorrect because it includes the letter U, which is not a nucleotide found in DNA. Choice D is incorrect because it includes 10 nucleotides instead of 9.

What Would Be the 9-Residue Primer Used to Amplify This