In the experiment shown in the figure above,Tonegawa and his colleagues were able to express β-globin in an antibody-producing cell that normally does not express β-globin.They achieved this result by splicing an enhancer from an antibody-producing gene into the protein-coding portion of the β-globin gene.They then introduced this recombinant gene into cultured antibody-producing cells.Why was the choice of antibody-producing cells rather than,say,muscle or skin cells critical for the success of this experiment?
A)Only the antibody-producing cells have the correct regulatory transcription factors to bind to the enhancer.
B)Only the antibody-producing cells have the correct set of enhancers.
C)Only the antibody-producing cells have the correct set of promoter-proximal elements.
D)Only the antibody-producing cells have the correct set of enhancers,promoter-proximal elements,and promoters.
E)Only the antibody-producing cells are capable of expressing recombinant genes.

Question

Quizplus · Accepted Answer

Only the antibody-producing cells have the correct regulatory transcription factors to bind to the enhancer. Antibody-producing cells have a specific set of transcription factors that are necessary for the production of antibodies. These transcription factors are not present in other types of cells such as muscle or skin cells. Therefore, the choice of antibody-producing cells was critical for the success of this experiment as only these cells have the necessary regulatory factors to express the recombinant gene.

In the Experiment Shown in the Figure Above,Tonegawa and His