The assumptions made in calculating the Michaelis-Menten Equation include
A)that the formation and decomposition of ES is the same for a period of time.
B)that the concentration of the substrate is much greater than the concentration of E.
C)that the value of k-2 can be ignored.
D)A,B and C.
E)A and B only.

Question

Quizplus · Accepted Answer

The Michaelis-Menten equation assumes that the formation and breakdown of the enzyme-substrate complex (ES) reach a steady state (A), that substrate concentration is much higher than enzyme concentration (B), and that the reverse reaction from product to ES (k-2) is negligible (C).

The Assumptions Made in Calculating the Michaelis-Menten Equation Include