The Km values for enzyme reactions such as A + B → C + D
A)cannot be determined using the Lineweaver-Burk plot analysis.
B)can be determined by holding one (A or B)at high concentration,while varying the concentration of the other substrate.
C)can be determined for one substrate and not the other.
D)do not indicate the efficiency of the enzyme.

Question

Quizplus · Accepted Answer

The Km value can be determined by holding one substrate at high concentration and varying the concentration of the other substrate. This is known as the method of initial rates, which involves measuring the rate of the reaction at different substrate concentrations and plotting the data as a Lineweaver-Burk plot to determine the Km value.

The Km Values for Enzyme Reactions Such as a