Whether libraries are cDNA or genomic,whether amplification is based on PCR or cloning,and whether screening is done with northern or Southern blotting or probes,all are based on which of the following?
A)complementarity
B)complementation
C)reverse transcription
D)reverse translation
E)gene expression

Question

Quizplus · Accepted Answer

All of the techniques mentioned rely on the principle of complementary base pairing between nucleic acid sequences in order to identify, amplify, or isolate specific genetic information. This includes complementary binding between probes and target sequences in Northern or Southern blotting, annealing of primers in PCR amplification, and hybridization of cDNA or genomic sequences in library construction. Complementation, reverse transcription, and reverse translation are not directly related to these techniques. Gene expression may be involved, but is not the underlying principle that all of these techniques are based on.

Whether Libraries Are CDNA or Genomic,whether Amplification Is Based on PCR