A mammalian expression cassette used to produce an exogenous gene in a virus vector must have which of the following components?

Question

Quizplus · Accepted Answer

All of the components listed are essential for the efficient expression of an exogenous gene in a mammalian expression system using a virus vector. Splicing signals are necessary for the removal of introns and proper processing of the mRNA. A strong enhancer increases the transcription levels of the gene. The polyadenylation signal is crucial for the stability and export of the mRNA from the nucleus. The translational leader sequence is important for the initiation of translation.

A Mammalian Expression Cassette Used to Produce an Exogenous Gene