A fluorophore used in fluorescence microscopy that absorbs light at 260 nm would most likely fluoresce at:
A) 100 nm
B) 200 nm
C) 260 nm
D) 400 nm
E) 800 nm

Question

Quizplus · Accepted Answer

A fluorophore that absorbs light at 260 nm belongs to a class of fluorophores called UV excitable fluorophores. These fluorophores typically have an emission maximum in the visible range, between 400-700 nm. Therefore, the most likely choice for the wavelength of fluorescence would be D (400 nm) as it falls within this range.

A Fluorophore Used in Fluorescence Microscopy That Absorbs Light at 260