The colorless molecule o-nitrophenyl galactoside (ONPG) can be used as an artificial substrate in assays of -galactosidase in E. coli extracts. Which of the following statements is NOT true with respect to the use of ONPG?
A) Hydrolysis of ONPG by -galactosidase produces yellow o-nitrophenol.
B) o-nitrophenol can be measured with a spectrophotometer.
C) Hydrolysis of ONPG by -galactosidase also releases colorless galactose.
D) The o-nitrophenol moiety is blue and more suitable for use in solid media.
E) None of the above.
Correct Answer:
Verified
Q9: Microbes that grow at neutral pH but
Q10: Which of the following is correct regarding
Q11: A common approach to study a certain
Q12: A significant advantage in real-time PCR is
Q13: For a reporter protein to be synthesized,
Q15: The following is correct regarding operon fusions:
A)
Q16: What kind of mutation is produced by
Q17: Sigma B is a stress-induced transcription
Q18: Infection of potato crops by the fungus
Q19: The genes for proteins such as
Unlock this Answer For Free Now!
View this answer and more for free by performing one of the following actions
Scan the QR code to install the App and get 2 free unlocks
Unlock quizzes for free by uploading documents