A Significant Advantage in Real-Time PCR Is That

A significant advantage in real-time PCR is that:

A) initial amounts of template DNA can be quantified by the length of time it takes to detect PCR products
B) amplified DNA is quantified while the PCR is still taking place
C) earlier increments in fluorescence indicate larger amounts of template DNA in the original sample
D) all of the above
E) none of the above