Which Is NOT True When Scaling Up Preparative Chromatography

Which is NOT true when scaling up preparative chromatography?

A) Keep column length constant.
B) Cross-sectional area of a column is inversely proportional to the mass of analyte.
C) Maintain constant linear velocity.
D) Sample volume applied to column is proportional to mass of analyte.
E) If you change column length,mass of sample can be increased in proportion to total length.