Gel filtration chromatography separates proteins on the basis of their:
A)charge.
B)mass.
C)affinity for a ligand.
D)mass and charge.

Question

Quizplus · Accepted Answer

Gel filtration chromatography separates proteins based on their size or mass. Larger proteins cannot enter the pores of the gel filtration column and therefore elute first, while smaller proteins are able to enter the pores and therefore elute later. This separation is not based on charge or affinity for a ligand.

Gel Filtration Chromatography Separates Proteins on the Basis of Their