In two-dimensional gel electrophoresis,proteins are first resolved by _____ and then by _____.
A)IEF;SDS-PAGE
B)SDS-PAGE;affinity chromatography
C)SDS-PAGE;ion exchange
D)IEF;gel filtration

Question

Quizplus · Accepted Answer

In two-dimensional gel electrophoresis, proteins are first separated by isoelectric focusing (IEF) based on their isoelectric points, and then by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) based on their molecular weights. Therefore, choice A (IEF;SDS-PAGE) is the correct answer.

In Two-Dimensional Gel Electrophoresis,proteins Are First Resolved by _____ and Then