A chunk of tissue is treated so that each cell's membrane is broken open to release the contents inside,and then subjected to differential centrifugation.Which of the following is true at the end of the centrifugation?
A)Depending on the speed of the centrifugation,the proteasome is more likely to be in the supernatant than a chaperone.
B)Proteins of similar density will be found in the same fraction (either pellet or supernatant).
C)The pellet contains the least dense material.
D)The pellet will contain a purified protein for further analysis.

Question

Quizplus · Accepted Answer

Differential centrifugation separates cellular components based on their densities. Proteins of similar densities will be found in the same fraction either pellet or supernatant. The speed of centrifugation does not determine whether a proteasome or a chaperone will be in the supernatant or pellet. The pellet may contain impurities and is not necessarily a purified protein. The pellet may contain dense material, such as organelles like mitochondria or nuclei that have been centrifuged to the bottom.

A Chunk of Tissue Is Treated So That Each Cell's