You are studying the expression of the gene that appears to be under the control of three different transcription-control regions during mouse embryonic development.Which one of the following is the BEST method to use to determine when each of these regions are active in the developing mouse embryo?
A)DNA affinity chromatography
B)polymerase chain reaction
C)reporter gene assay
D)DNAse 1 footprinting

Question

Quizplus · Accepted Answer

Reporter gene assays involve fusing a reporter gene (e.g. luciferase) to the regulatory regions of a gene of interest. The activity of the reporter gene reflects the activity of the regulatory regions. By using different versions of the gene construct with different regulatory regions and introducing them into mouse embryos at different developmental stages, the activity of each regulatory region can be determined. This method is non-invasive and allows for monitoring of gene expression over time.

You Are Studying the Expression of the Gene That Appears