You perform an experiment where you prevent Cdc20 from binding to APC
In a cell line that usually completes the cell cycle in 60 minutes.Your experiment prevents Cdc20 from binding to APC for 120 minutes,and then you fix and stain the DNA,securin,separase and cohesin proteins.Which of the following would NOT be found in the observed cell?
A)The cell would contain condensed chromosomes aligned at the metaphase plate.
B)Cohesins will be localized around the sister chromatids.
C)Securin would be bound to separase (appear to co-localize).
D)The DNA will be decondensed because the cell is in interphase.

Question

Quizplus · Accepted Answer

If Cdc20 is prevented from binding to APC for twice the length of the normal cell cycle, the cell would likely be arrested in metaphase, with condensed chromosomes aligned at the metaphase plate. Cohesins would still be localized around the sister chromatids, and securin would still be bound to separase, appearing to co-localize. However, the DNA would not be decondensed because the cell would not have progressed back to interphase.

You Perform an Experiment Where You Prevent Cdc20 from Binding