In vertebrates,the family of myogenic regulatory factors (MFRs)plays a major role in muscle development.This family consists of four related genes,including MyoD,MRF4,myogenin,and myf5,each of which have distinct,but overlapping functions.Interestingly,this gene family is represented by only a single gene called nautilus in Drosophila.You want to determine if nautilus functions as the sum of the vertebrate factors,or rather,if it functions in a manner similar to that of one particular gene.Eventually,you plan to examine the loss-of-function phenotype of nautilus mutants.However,first you should
A)compare the DNA and protein sequences of all the genes to determine if nautilus is more closely related to one MRF than another.
B)perform a DNA microarray to compare gene activation patterns between nautilus and the MRFs.
C)compare the proteome of a cell expressing nautilus to the proteomes of cells expressing each of the MRFs in turn.
D)generate four transgenic Drosophila strains that express the MRFs.

Question

Quizplus · Accepted Answer

Comparing the DNA and protein sequences of all the genes can provide information on the similarity between nautilus and each of the MRFs. This can help determine if nautilus functions as the sum of the vertebrate factors, or if it has a more similar function to one specific MRF. This information can guide the subsequent experiments, including examining the loss-of-function phenotype of nautilus mutants. Performing DNA microarrays or comparing proteomes can also provide useful information, but may not directly address the question of nautilus' similarity to the MRFs. Generating transgenic Drosophila strains that express the MRFs may not provide relevant information to determine nautilus' function.

In Vertebrates,the Family of Myogenic Regulatory Factors (MFRs)plays a Major