You are performing a yeast two hybrid screen to identify protein-protein interactions.Your bait is a 25 kD protein fused to the GAL4 DNA-binding domain.Which of the following best describes the nature of the library that you are using?
A)The library contains the prey fused to the GAL4 activation domain.
B)The library contains the reporter gene fused to the GAL4 activation domain.
C)The library contains the bait fused to the GAL4 activation domain.
D)The library contains the bait fused to the reporter gene,which is upstream of the GAL4 activation domain.

Question

Quizplus · Accepted Answer

In yeast two hybrid screening, the library typically contains a collection of prey proteins fused to the GAL4 activation domain. The bait protein, in this case a 25 kD protein fused to the GAL4 DNA-binding domain, is used to identify interacting proteins from the library. The interaction between the bait and prey proteins reconstitutes the transcriptional activator complex that activates expression of the reporter gene, which is typically located upstream of the GAL4 activation domain. Therefore, choice A is the best choice as it correctly describes the nature of the library that would be used in this type of experiment.

You Are Performing a Yeast Two Hybrid Screen to Identify