To generate a transgenic mouse,one would
A)introduce the knocked-out gene into a blastocyst embryo,isolate the embryonic stem cells,implant the embryonic stem cells into a pseudopregnant female.
B)introduce the knocked-out gene into embryonic stem cells,isolate the cells from a pseudopregnant female,inject the transformed cells into a blastocyst stage embryo.
C)introduce the knocked-out gene into embryonic stem cells,implant the embryonic stem cells into a pseudopregnant female.
D)introduce the knocked-out gene into embryonic stem cells,inject the embryonic stem cells into a blastocyst stage embryo,implant the injected blastocyst into a pseudopregnant female.

Question

Quizplus · Accepted Answer

The most common method to generate a transgenic mouse is to inject genetically modified embryonic stem cells into a blastocyst stage embryo, which is then implanted into a pseudopregnant female. This ensures that the modified gene is present in every cell of the developing mouse, leading to a transgenic mouse with the desired trait. Option D describes this process accurately. Option A describes the method for generating a knockout mouse, which involves deleting a specific gene rather than introducing a new gene. Option B is not a common method for generating transgenic mice, as it involves isolating the modified cells from a pseudopregnant female, which can be difficult and time-consuming. Option C is also not a common method for generating transgenic mice, as introducing the modified embryonic stem cells into a pseudopregnant female without the blastocyst stage embryo may result in low efficiency of embryo development.

To Generate a Transgenic Mouse,one Would