Which of the following is not used in PCR amplification of DNA?
A)Double-stranded DNA template
B)Oligonucleotide primers
C)DNA polymerase
D)Buffer
E)ddNTPs

Question

Quizplus · Accepted Answer

ddNTPs (dideoxynucleotides) are not used in PCR amplification. They are used in Sanger sequencing to terminate DNA chain elongation. PCR amplification requires the use of a double-stranded DNA template, oligonucleotide primers, DNA polymerase, and buffer.

Which of the Following Is Not Used in PCR Amplification