Which of the following does not occur during PCR?
A)Denaturation at high temperatures
B)Synthesis of oligonucleotide primers
C)Annealing of oligonucleotide primers
D)Extension of primers by DNA polymerase
E)Changes in the reaction temperature by a thermocycler

Question

Quizplus · Accepted Answer

Synthesis of oligonucleotide primers is not a step that occurs during PCR. The primers used in PCR are typically synthesized beforehand and added to the reaction mixture. The other steps listed (denaturation, annealing, extension, temperature changes) are all essential components of the PCR process.

Which of the Following Does Not Occur During PCR