If you are setting up a PCR reaction,how much extension time per cycle should you allocate for the Taq DNA polymerase to amplify a 5-kb DNA fragment from a 10-kb template?
A)1 minute
B)2.5 minutes
C)5 minutes
D)10 minutes
E)20 minutes

Question

Quizplus · Accepted Answer

The general rule of thumb is to allocate approximately 1 minute of extension time per kb of template DNA. Therefore, for a 5-kb DNA fragment from a 10-kb template, 5 minutes of extension time per cycle should be allocated for the Taq DNA polymerase to amplify the template. Allocating more extension time than necessary can lead to non-specific amplification or the formation of undesired products.

If You Are Setting Up a PCR Reaction,how Much Extension