Certain immunoassays require separation of bound reactant from free-labeled reactant for determination of analyte concentration.Which one of the following immunoassays requires this separation step?

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ELISA is the only immunoassay listed that requires separation of bound and free reactants for determination of analyte concentration. This is typically done using a washing step to remove unbound labels before measuring the signal. FPIA measures polarization of fluorescently-labeled reactants, EMIT measures the amount of fluorescence emitted by labeled reactants, and Cloned enzyme donor immunoassay measures the activity of an enzyme reporter fused to the analyte-specific antibody. None of these methods require separation of bound and free reactants.

Certain Immunoassays Require Separation of Bound Reactant from Free-Labeled Reactant