In a noncompetitive immunoassay reaction format:

Question

Quizplus · Accepted Answer

In a noncompetitive immunoassay, the choice C best describes the format. The assay involves allowing antigen from an unknown sample to react with a solid-phase antibody, followed by the addition of a second labeled antibody that reacts with bound antigen. This format enables the detection of the antigen-antibody complex formed by the unknown sample and the solid-phase antibody, resulting in the measurement of the target antigen. Choice A is incorrect because in this format, both the labeled and unlabeled antigens bind to the antibody, resulting in competition for binding sites. Choice B describes a competitive immunoassay, not a noncompetitive format. Choice D is not a characteristic of a noncompetitive immunoassay.

In a Noncompetitive Immunoassay Reaction Format