A homogeneous fluorescence immunoassay that involves the speed of the rotation of molecules,which is then related to analyte concentration in a patient sample,is known as:

Question

Quizplus · Accepted Answer

The description given in the question matches the principle of fluorescence polarization immunoassay. In this assay, a fluorescent molecule is tagged to the analyte-specific antibody, and when the sample is excited with polarized light, the rate of rotation of the fluorescent molecule changes based on the presence or absence of analyte. Higher analyte concentration in the sample causes a decrease in fluorescence polarization, which is then measured and correlated with the concentration of analyte. ELISA, reflectance immunoassay, and enzyme-multiplied immunoassay technique use different principles for analyte detection.

A Homogeneous Fluorescence Immunoassay That Involves the Speed of the Rotation