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In a Noncompetitive Immunoassay Reaction Format,a Very High Concentration of Analyte

Question 2

Multiple Choice

In a noncompetitive immunoassay reaction format,a very high concentration of analyte antigen in the unknown sample might produce a falsely:


A) decreased result because the high concentration of antigen binds both the capture and labeled antibody,reducing the number of complexes formed.
B) increased result because the rate of the forward reaction of antigen-antibody binding is too fast,leading to a high equilibrium constant.
C) decreased result because of the free excess antigen-binding all antibody sites and not allowing any unlabeled antigen to bind.
D) increased result because the second labeled antibody will be able to react with the substrate by itself since excess antigen will interfere with any binding.

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