You have prepared a restriction enzyme digest of DNA from an individual who might have a genetic disorder.On the gel,you note that the digest from this individual produces one fragment,whereas the digest from a normal healthy individual produces two fragments.All controls worked correctly and you used the same reagents for all testing.What is your interpretation?

Question

Quizplus · Accepted Answer

The fact that the digest from the diseased individual produces one fragment while the normal individual produces two fragments indicates that the restriction site is missing in the diseased individual's DNA. This suggests a possible genetic mutation or disorder that affects the DNA sequence where the restriction site is located. The controls working correctly and the use of the same reagents for all testing rules out the possibility of the enzymes not working or skipping the amplification step. The fact that the normal individual's DNA produces two fragments suggests that the restriction site is present in their DNA, ruling out option D.

You Have Prepared a Restriction Enzyme Digest of DNA from an Individual