If RNA is to be used in a PCR amplification procedure,what is the initial step that must be performed?

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Quizplus · Accepted Answer

RNA cannot be directly used in PCR because thermostable DNA polymerases cannot bind to RNA and synthesize a complementary strand. Therefore, the first step in using RNA in a PCR reaction would be to perform reverse transcription, which involves using a reverse transcriptase enzyme to synthesize a cDNA (complementary DNA) copy of the RNA template. This cDNA can then be used as the template for the PCR reaction.

If RNA Is to Be Used in a PCR Amplification