A fast and common method for determining the protein concentration in column effluent is
A)tandem mass spectrometry.
B)salting in with ammonium sulfate.
C)drying a portion and weighing the solid.
D)measuring light absorption at 280 nm.
E)Edman degradation.

Question

Quizplus · Accepted Answer

Measuring the absorbance of light at 280 nm is a common and fast method for determining protein concentration in column effluent. This method takes advantage of the fact that proteins absorb light at this wavelength due to the presence of aromatic amino acids such as tryptophan and tyrosine.

A Fast and Common Method for Determining the Protein Concentration