Matching

-______ chromatography is a method of fractionating a protein mixture according to differences in polarity.
A) electrophoresis
B) hydrophobic interaction
C) enzyme-linked immunosorbent assay
D) three-dimensional shape
E) N-terminal amino acid
F) negative charge
G) nucleases
H) chromophore
I) foaming
J) high level expression
K) 2-mercaptoethanol
L) positive charge
M) cation exchange
N) pI
O) chymotrypsin
P) C-terminal amino acid
Q) Sodium dodecyl sulfate

Question

Matching

-______ chromatography is a method of fractionating a protein mixture according to differences in polarity.
A) electrophoresis
B) hydrophobic interaction
C) enzyme-linked immunosorbent assay
D) three-dimensional shape
E) N-terminal amino acid
F) negative charge
G) nucleases
H) chromophore
I) foaming 
J) high level expression
K) 2-mercaptoethanol
L) positive charge
M) cation exchange
N) pI
O) chymotrypsin
P) C-terminal amino acid
Q) Sodium dodecyl sulfate

Quizplus · Accepted Answer

Hydrophobic interaction chromatography separates proteins based on their hydrophobicity, which is related to their polarity. Proteins with more hydrophobic surfaces will interact more strongly with the hydrophobic stationary phase and thus can be fractionated according to their differences in polarity.

Matching -______ Chromatography Is a Method of Fractionating a Protein Mixture