A technique that can be used to separate proteins based primarily on the presence of non-polar residues on their surface is called
A)ion-exchange chromatography.
B)gel filtration chromatography.
C)affinity chromatography.
D)gel electrophoresis.
E)hydrophobic interaction chromatography.

Question

Quizplus · Accepted Answer

Hydrophobic interaction chromatography is a technique used to separate proteins based on the hydrophobicity of their surface. Proteins with more non-polar residues on their surface interact more strongly with the hydrophobic stationary phase, and are eluted later than proteins with fewer non-polar residues. The other options listed do not rely primarily on the hydrophobicity of the protein's surface for separation.

A Technique That Can Be Used to Separate Proteins Based