A first step in purifying a protein that was initially associated with fatty substances would be
A)Coomassie Brilliant Blue dye staining.
B)analytical ultracentrifugation.
C)ELISA.
D)Western blotting.
E)hydrophobic interaction chromatography.

Question

Quizplus · Accepted Answer

Hydrophobic interaction chromatography would be the best choice for purifying a protein that was initially associated with fatty substances because the technique separates proteins based on their hydrophobicity, which could be influenced by the presence of fatty substances.

A First Step in Purifying a Protein That Was Initially