You are trying to separate five proteins,which are listed below,by gel filtration chromatography.Which of the proteins will elute first from the column?
A)cytochrome c (12 kDa)
B)RNA polymerase (99 kDa)
C)glutamine synthetase (621 kDa)
D)interferon-$\gamma$ (34 kDa)
E)hemoglobin (62 kDa)

Question

Quizplus · Accepted Answer

In gel filtration chromatography, molecules are separated based on their size, with larger molecules eluting first because they cannot enter the pores of the gel and thus bypass the gel matrix more quickly. Glutamine synthetase, being the largest molecule listed at 621 kDa, will elute first from the column.

You Are Trying to Separate Five Proteins,which Are Listed Below,by γ\gammaγ

You Are Trying to Separate Five Proteins,which Are Listed Below,by $\gamma$