What can be done to increase the rate at which a protein of interest moves down an ion-exchange chromatography column?
A)reduce the ion concentration in the eluant
B)add a small amount of a non-ionic detergents to the eluant
C)change the pH of the eluant
D)add a protease inhibitor to the eluant
E)reduce the temperature of the eluant

Question

Quizplus · Accepted Answer

Changing the pH of the eluant can alter the charge of the protein, affecting its interaction with the ion-exchange resin and potentially increasing its rate of movement through the column.

What Can Be Done to Increase the Rate at Which