Which of the following amino acids would be first to elute at pH 8.0 from an anion-exchange column?
A)lysine
B)alanine
C)glutamic acid
D)asparagine
E)glycine

Question

Quizplus · Accepted Answer

At pH 8.0, lysine, which is a basic amino acid, will have a positive charge and thus will not bind or will be weakly bound to an anion-exchange column, leading it to elute first. Anion-exchange columns bind negatively charged molecules, and positively charged molecules elute first.

Which of the Following Amino Acids Would Be First to Elute