Which of the following amino acids would be last to elute at pH 8.0 from an anion-exchange column?
A)lysine
B)alanine
C)glutamic acid
D)asparagine
E)glycine

Question

Quizplus · Accepted Answer

At pH 8.0, glutamic acid will have a net negative charge due to the deprotonation of its side chain carboxyl group, making it bind more strongly to an anion-exchange column. Amino acids with net negative charges elute later from an anion-exchange column because they are attracted to the positively charged groups on the resin.

Which of the Following Amino Acids Would Be Last to Elute