A technique that can be used to separate proteins based primarily on their pI is called
A)ion-exchange chromatography.
B)gel filtration chromatography.
C)affinity chromatography.
D)isoelectric focusing.
E)hydrophobic interaction chromatography.

Question

Quizplus · Accepted Answer

Isoelectric focusing separates proteins based on their isoelectric point (pI), which is the pH at which the protein has no net charge and will not move in an electrical field. Proteins are loaded onto a gel or strip with a pH gradient, and an electric current is applied. Proteins move until they reach their pI, where they become neutral and stop migrating. The separated proteins can then be visualized and collected for further study.

A Technique That Can Be Used to Separate Proteins Based