Which of the following PCR controls ensures that the reaction mix is not contaminated with amplicons from a previous PCR procedure and must not have a PCR product detected in order to be valid?

Question

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Reagent blank is the control that ensures that the reaction mix is not contaminated with amplicons from a previous PCR procedure, as it contains all the reagents used for the reaction except for the template DNA. If there is amplification detected in the reagent blank, it suggests contamination in the reagents or PCR set-up. Positive control confirms that the PCR reaction is working properly and must have a PCR product detected in order to be a valid control. Sensitivity control evaluates the limit of detection of the PCR assay. Amplification control checks the presence of inhibitory substances in the template DNA or reaction mix.

Which of the Following PCR Controls Ensures That the Reaction