Which of the following is the major disadvantage of RT-PCR?

Question

Quizplus · Accepted Answer

The major disadvantage of RT-PCR is that RNA is labile and requires careful handling to avoid degradation by RNases. DNA-based testing also requires meticulous care to avoid degradation, but this is not the major disadvantage of RT-PCR. Large sample sizes are not required for RT-PCR, and it is actually a highly sensitive technique that can detect low numbers of target sequences. Therefore, options A, B, and C are incorrect.

Which of the Following Is the Major Disadvantage of RT-PCR