Refer to the table below. The enzyme alcohol dehydrogenase was purified from yeast cells.It was then dialyzed against a buffer solution, which means that it was placed inside a bag consisting of a semipermeable membrane that allowed small molecules and ions to pass through, but not the enzyme.The enzyme was removed from the dialysis bag, incubated with the compound N-ethylmaleimide, and then dialyzed a second time.At each step, the enzyme's catalytic activity was measured and expressed as a percentage of its original activity shown in the table.Which statement expresses a valid conclusion that can be made based on these results?

Question

Refer to the table below.  The enzyme alcohol dehydrogenase was purified from yeast cells.It was then dialyzed against a buffer solution, which means that it was placed inside a bag consisting of a semipermeable membrane that allowed small molecules and ions to pass through, but not the enzyme.The enzyme was removed from the dialysis bag, incubated with the compound N-ethylmaleimide, and then dialyzed a second time.At each step, the enzyme's catalytic activity was measured and expressed as a percentage of its original activity shown in the table.Which statement expresses a valid conclusion that can be made based on these results?

Quizplus · Accepted Answer

The fact that after the enzyme was incubated with N-ethylmaleimide, its catalytic activity dropped to 0%, suggests that the inhibition is irreversible. Therefore, the best choice is E, N-ethylmaleimide is an irreversible inhibitor of alcohol dehydrogenase. Choices A, B, C, and D do not fit the results presented in the table.

Refer to the Table Below