If the end of one of the sequences that you wish to amplify by PCR begins with TGTCGAT, the primer should _______ with _______.Note: All sequences are shown in the 5'-to-3' orientation.
A) begin; ACAGCTA
B) begin; TGTCGAT
C) end; ACAGCTA
D) end; TGTCGAT
E) end; AAAAAAA

Question

Quizplus · Accepted Answer

Primers for PCR should be complementary and antiparallel to the target sequence. Since the sequence to be amplified ends with TGTCGAT, the primer should end with the complementary sequence ACAGCTA to bind effectively.

If the End of One of the Sequences That You