In PCR, the temperature is lowest when
A) DNA is separated into two strands.
B) primers anneal with the template.
C) DNA polymerase is producing new strands of DNA.
D) DNA is being repaired.
E) the temperature is the same during all of the stages of PCR.

Question

Quizplus · Accepted Answer

The temperature is lowest when primers anneal with the template. This is typically around 55-60°C in PCR, as it is the optimal temperature for primer binding. When the temperature is too high, the primers won't bind efficiently to the template, and when it is too low, the primers may bind less specifically to other regions of the DNA, leading to non-specific amplification.

In PCR, the Temperature Is Lowest When