Variation at a specific gene is known to affect metabolism in mice, but it is not clear which of the nucleotides is responsible for the changes.Which technique would be most relevant for revealing which of the nucleotides affects metabolism?
A) Microarrays
B) Antisense RNA
C) RNAi
D) Artificial synthesis
E) CRISPR

Question

Quizplus · Accepted Answer

CRISPR can be used to create specific mutations in the gene of interest, one at a time, to determine which nucleotide(s) affect metabolism. This approach is commonly used in functional genomics to identify the causal relationship between genotype and phenotype.

Variation at a Specific Gene Is Known to Affect Metabolism