It is suspected that the gene DEFT regulates the expression of many other genes.How could one test this?
A) Knock out different genes using CRISPR, then see how DEFT is expressed using microarrays.
B) Knock out different genes using CRISPR, then see how DEFT is expressed using RNAi.
C) Use CRISPR to knock DEFT out, then use microarrays to examine how genes are expressed with and without DEFT expression.
D) Use CRISPR to knock DEFT out, then use RNAi to examine how genes are expressed with and without DEFT expression.
E) Use microarrays to knock out DEFT, then use RT-PCR to see how genes are expressed with and without DEFT expression.

Question

Quizplus · Accepted Answer

Using CRISPR to knock out the DEFT gene and then examining the expression of other genes using microarrays will reveal which genes are affected by DEFT expression. This will allow us to determine whether DEFT regulates the expression of many other genes. Using RNAi instead of CRISPR would not provide conclusive results, as RNAi only suppresses gene expression rather than completely knocking out the gene. Similarly, knocking out other genes using CRISPR does not directly address the role of DEFT in regulating gene expression. Microarrays cannot be used to knock out genes, so option E is not feasible.

It Is Suspected That the Gene DEFT Regulates the Expression