Which sequence is least likely to be a palindromic site recognized by a restriction enzyme?
A) 5ʹ-GTAC-3ʹ
B) 5ʹ-AGCT-3ʹ
C) 5ʹ-AGTACT-3ʹ
D) 5ʹ-CTGAAG-3ʹ
E) 5ʹ-TGTACA-3ʹ

Question

Quizplus · Accepted Answer

A palindromic sequence is the same when read forward and backward, such as 5ʹ-GAATTC-3ʹ or 5ʹ-AGCT-3ʹ. Option D (5ʹ-CTGAAG-3ʹ) is not palindromic as it reads different when read backward (5ʹ-GAAGTC-3ʹ), making it the least likely to be recognized by a restriction enzyme that cuts at a palindromic site.

Which Sequence Is Least Likely to Be a Palindromic Site