The DNA polymerase reaction is a nucleophilic substitution facilitated by:
A) the 3'OH group being a weak nucleophile.
B) the 3'OH group being a strong nucleophile.
C) the pyrophosphate being a good leaving group.
D) the reaction being irreversible.
E) none of the above.

Question

Quizplus · Accepted Answer

The pyrophosphate is a good leaving group because it is a highly charged anion. This makes it a good nucleophile for the 3'OH group, which is a weak nucleophile.

The DNA Polymerase Reaction Is a Nucleophilic Substitution Facilitated By