Which of the following statements about E. coli DNA replication is INCORRECT?
A) The β subunit of DNA polymerase III is the sliding clamp essential for processivity.
B) The clamp-loading complex uses exchange between ADP and ATP to eject the clamp loader and close the clamp.
C) Single-stranded DNA-binding protein promotes denaturation of DNA by lowering the melting temperature.
D) Helicases are multimeric proteins that bind preferentially to one strand and rely on ATP-hydrolysis to unwind the duplex.
E) Topoisomerases are responsible for relieving torsional stress ahead of the replication fork generated by unwinding the duplex.
Correct Answer:
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