A scientist does not have an antibody to detect the protein of interest, but wants to know if the gene is expressed at low levels. What technique could the researcher use to detect, but not quantify the expression of the gene of interest?
A) Western Blotting
B) real-time PCR
C) cloning
D) reverse transcriptase PCR

Question

Quizplus · Accepted Answer

Reverse transcriptase PCR (RT-PCR) can be used to detect the presence of RNA transcripts from the gene of interest, even if they are present at low levels. This technique involves reverse transcribing RNA into cDNA, which can then be amplified using PCR. The resulting PCR products can be visualized on an agarose gel to confirm the presence of the gene of interest.

A Scientist Does Not Have an Antibody to Detect the Protein