Question 1

A hypothetical restriction sequence is 5′-TCTAGA-3′. The palindromic sequence is 3′-________-5′.

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Question 2

What technique of genomic library construction allows the genome to be cut by a given restriction enzyme at some but not all recognition sequences?

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Question 3

In a cDNA library, not all expressed genes will be represented. On what does this depend?

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Question 4

In the formation of transgenic animals, DNA is usually inserted into eggs or embryos. If some cells of the embryo do not receive the introduced DNA, the result is an organism with cells of different genotypes; the organism is known as what?

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Question 5

In the case of Jesse Gelsinger, a young man who died during gene therapy, the therapy involved delivery via a viral vector. Was this germ-line or somatic therapy?

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Question 6

By what method is T-DNA integrated into the plant genome?

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Question 7

One way to genetically engineer S. cerevisiae is by homologous recombination. If a given sequence is thus introduced and is integrated via two regions of homology, is it more probably introduced as a linear or as a circular entity?

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Question 8

In creating a recombinant DNA molecule, what are two methods that prevent re-ligation of the cloning vector that contains no insert DNA?

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Question 9

Specific sequences in the $\lambda$ genome that interact with coat proteins to package genomes into phage particles are known as ________ sites.

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Question 10

In the pUC plasmids, a polylinker region that includes multiple restriction sites is embedded in the lacZ component. If a sequence of interest is inserted into this region, what is the effect on lacZ?

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Question 11

If a gene is replaced with a heterologous sequence such that the gene is then nonfunctional, what is the result usually called?

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Question 12

If a researcher is cloning a sequence by using pUC and uses the plasmid to transform bacteria, which are then plated on X-gal medium, would the recombinant plasmids be found in blue or white colonies or both?

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Question 13

Snuppy, a male Afghan hound, was created by transferring the nucleus from an ear cell of the male Afghan, Tai, into an enucleated egg from a donor. The embryo was transplanted into a surrogate mother to develop to term. Which of the following animals would be a genetic match for Snuppy if mitochondrial DNA were tested and if Snuppy were not a clone, but the product of in vitro fertilization?

Accepted Answer

Mitochondrial DNA is inherited maternally, so the egg donor would be the source of Snuppy's mitochondrial DNA. Because Snuppy was created by transferring the nucleus from Tai's cell into the egg donor's enucleated egg, Tai's mitochondrial DNA would not be present in Snuppy. The surrogate would also not contribute to Snuppy's mitochondrial DNA.

Question 14

In grape cultivation, growers often find that heterozygous forms are more desirable. To propagate the heterozygotes without having to interbreed in each generation, what method can they use?

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Question 15

Which type of DNA library (genomic or cDNA)would include introns and promoters?

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Question 16

How can you use the known human cDNA gene sequence for cytC that you already have in an experiment(s)to identify and compare the sequences from other vertebrates?

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Question 17

Agrobacterium tumefaciens is often used in the production of transgenic plants because of its Ti plasmid. This plasmid is usually "disarmed" by deleting its tumor genes along with the genes for producing what products that would have been advantageous to the bacterium?

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Question 18

For a transgenic animal line to be produced from a chimera, the transgene must be carried by what?

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Question 19

In producing an E. coli plasmid to be an expression vector, an experimenter includes genes for selectable markers and a promoter for the expression of the introduced genes. She uses a cDNA sequence to ensure the insert contains no introns. The gene inserted is transcribed, but not translated. What sequence did she omit from the plasmid?

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Question 20

If a plant species cannot readily have a new gene integrated into it via a Ti plasmid, it must then be subjected to different methods. These often include removing the cell wall and inserting DNA, after treating the membrane with heat or electricity to do what to the membrane?

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Quiz 17: Applications of Recombinant Dna Technology and Reverse Genetics